Journal: International Journal of Molecular Sciences
Article Title: Artificial Intelligence Reveals Nature: Functional Parallels Between a Designed and a Natural Peptide
doi: 10.3390/ijms262110607
Figure Lengend Snippet: RBD-ACE2 interaction inhibition assays. ( a ) Schematic illustration of ELISA experiment to evaluate the ability of the A13 or Nat1 to prevent the interaction of SARS-CoV-2 S1 protein RBD to human receptor ACE2. First the plates were incubated with immobilized RBD. They were then inoculated with peptides prior to the addition of recombinant human receptor ACE2. ( b ) Inhibition of RBD-ACE2 binding via A13 or Nat1. The level of RBD-ACE2 binding was measured by chemiluminescence. The negative control with no peptide representing RBD-ACE2 interaction was set to 1.00 (3.2 × 10 4 RLU) and other values were normalized to this number. To the same extent, at the concentration of 0.1 µg/mL, both peptides reduced ACE2 binding compared to the control. At 1.0 µg/mL, inhibition was further enhanced. The negative control peptide did not exhibit a notable inhibition. Data represent the mean value from at least three independent experiments. Error bars represent standard deviation. Statistical significance was determined using the nonparametric two-tailed Student’s t -test (**** p ≤ 0.0001).
Article Snippet: The ability of the peptides to block the interaction between the RBD and ACE2 was assessed using a commercial ACE2 inhibition assay kit (BPS Bioscience, San Diego, CA, United States, CAT# 79931).
Techniques: Inhibition, Enzyme-linked Immunosorbent Assay, Incubation, Recombinant, Binding Assay, Negative Control, Concentration Assay, Control, Standard Deviation, Two Tailed Test